The mechanism of enzymatic synthesis of galactosyl oligosaccharides.

The Enzymatic Synthesis and Disproportionation of Galactosyl Oligosaccharides

Enzymatic Synthesis of Oligosaccharides Containing Ga 16 . 4 Gal Non - Reducing End Using ys - Galactanase from PeniciMum citrinum

The transglycesylation reaction was done with a fi-ga)actanase from Penieillium eitrinum. The regioseiectivity in the transglycosylation reaction was studied using soy bean arabinogalactan as a donor and monoeT disaccharide derivatives containing fi-galactosyl residue as accepters. We also synthesized oligosaccharides eontaining Galfi1.4Gal sequence such 2s Galfi1.4GalPl.4G]c, Galfi1.4Galfi1. 3...

Chain Elongation of raffinose in pea seeds. Isolation, characterization, and molecular cloning of mutifunctional enzyme catalyzing the synthesis of stachyose and verbascose.

Raffinose oligosaccharides are major soluble carbohydrates in seeds and other tissues of plants. Their biosynthesis proceeds by stepwise addition of galactose units to sucrose, which are provided by the unusual donor galactinol (O-alpha-d-galactopyranosyl-(1-->1)-l-myo-inositol). Chain elongation may also proceed by transfer of galactose units between raffinose oligosaccharides. We here report ...

Galactosyl-oligosaccharide formation during lactose hydrolysis: a review

Enzymatic hydrolysis of lactose is accompanied by galactosyl transfer to other sugars, thereby producing oligosaccharides. These are hydrolyzed slowly, both in vitro and in vivo. They can be thought of as low molecular weight, non-viscous, water-soluble, dietary fibre. They are considered to be physiologically functional foods which promote the growth of bifidobacteria in the colon and a wide v...

Synthesis of Oligosaccharides Derived from Lactulose (OsLu) Using Soluble and Immobilized Aspergillus oryzae β-Galactosidase

β-Galactosidase from Aspergillus oryzae offers a high yield for the synthesis of oligosaccharides derived from lactulose (OsLu) by transgalactosylation. Oligosaccharides with degree of polymerization (DP) ≥ 3 have shown to possess higher in vitro bifidogenic effect than di- and tetrasaccharides. Thus, in this work, an optimization of reaction conditions affecting the specific selectivity of A. ...